human siga  (Elabscience Biotechnology)

Journal: bioRxiv

Article Title: Isolation of Secretory Immunoglobulin A (sIgA) from nasal secretions collected in saline and Viral Transport Medium

doi: 10.1101/2025.06.26.661459

Figure Lengend Snippet: Protein from nasal secretions collected in different media were subjected to Western blot analysis. (A) Silver-stained gel; (B) Western blot of proteins (low concentration) from nasal secretions probed with anti-α heavy chain of sIgA; (C) Western blot of proteins (high concentration) from nasal secretions probed with anti-α heavy chain of sIgA. Lanes 1&2: sIgA from nasal secretions in saline; Lanes 3&4: sIgA from nasal secretions in VTM; Lanes 5&6: sIgA from defatted milk.

Article Snippet: The blot was incubated with biotinylated goat anti-human α chain of sIgA (5 μg/ml, Mabtech, Sweden) for 2 hr at RT.

Techniques: Western Blot, Staining, Concentration Assay, Saline

Journal: Microorganisms

Article Title: Bacteriome Signature in SARS-CoV-2-Infected Patients Correlates with Increased Gut Permeability and Systemic Inflammatory Cytokines

doi: 10.3390/microorganisms13061407

Figure Lengend Snippet: Zonulin plasma concentrations ( A ), and fecal secretory IgA ( B ) in control subjects (CTL), acute COVID-19 patients, and post-COVID-19 condition (PCC) patients.

Article Snippet: Fecal secretory immunoglobulin A (sIgA) was quantified by the commercial Human IgA ELISA kit (Elabscience, Bethesda, MD, USA), according to the manufacturer’s protocol.

Techniques: Clinical Proteomics, Control

Journal: Microorganisms

Article Title: Bacteriome Signature in SARS-CoV-2-Infected Patients Correlates with Increased Gut Permeability and Systemic Inflammatory Cytokines

doi: 10.3390/microorganisms13061407

Figure Lengend Snippet: Correlations between taxonomic counts of the gut bacteriome and clinical and laboratory data in COVID-19 patients. ( A ) C-reactive protein with differentially increased genera. ( B , C ) Plasma zonulin levels with differentially increased families and genera. ( D , E ) Fecal sIgA levels with differentially increased Bacillota, Prevotellacea, and some genera. ( F – H ) Plasma concentrations of IL-6, IL-2 and IFN-γ with differentially increased taxa.

Article Snippet: Fecal secretory immunoglobulin A (sIgA) was quantified by the commercial Human IgA ELISA kit (Elabscience, Bethesda, MD, USA), according to the manufacturer’s protocol.

Techniques: Clinical Proteomics

Journal: Journal of Functional Foods

Article Title: Effects of the colostrum-containing multi-nutrient dietary supplement on immune, muscle, bone, and cognitive function parameters in the elderly: A randomized, double-blind, placebo-controlled trial

doi: 10.1016/j.jff.2025.106816

Figure Lengend Snippet: Fig. 2. Changes in the levels of immunoglobulins following a 12-week intervention in the COL group and the PLA group. (A) stool bovine IgG, (B) saliva human sIgA, and (C) serum human IgG. Data expressed are the mean change from baseline ± SD. #p < 0.05, compared with baseline. *p < 0.05, compared between the COL group and the PLA group. Abbreviations – COL, colostrum-containing dietary supplement product; IgG, immunoglobulin G; PLA, placebo; sIgA, secretory immunoglob ulin A.

Article Snippet: Commercial enzyme-linked immunosorbent assay (ELISA) kits were used, according to the manufacturer’s instructions, to assess levels of stool bovine IgG (FineTest, cat. EB0196, Wuhan, China), saliva human sIgA (Elabscience, cat. E-EL-H1275, Houston, TX, USA), serum human IgG (Elabscience, cat. E-UNELH0079), serum human IL-6 (Elabscience, cat. E-UNEL-H0097), TNF-α (Elabscience, cat. E-UNEL-H0175), and IL-10 (Elabscience, cat. E-UNELH0084).

Techniques: